Abstract: Objective To explore the genetic polymorphisms of 19 STR loci of the Pembroke Welsh Corgi (Hereinafter referred to as corgi) . Method The PCR amplification was performed using the 19 STR fluorescent multiple amplification kit for dog. The amplified products were detected and statistically analyzed. Result To analysis the genetic polymorphisms of 19 loci of 212 purebred corgis, using HardyWeinberg equilibrium testing, all loci had P values greater than 0. 05 except PEZ20,the Cumulative PE (CPE) is 0. 9998289783352165 and the Total Power of Discrimination ( TDP ) is 0. 999999999999998 by 18 STR loci, the average Heterozygosity is 0. 631 and the average Polymorphism Information Content is 0. 604. Conclusion 18 of the 19 STR loci ( except PEZ20 ) can be applied to individual discrimination and paternity test of corgi.

Key words: corgi, STR, genetic polymorphism, paternity test, individual discrimination

摘要： 目的 对彭布洛克威尔士柯基犬(以下简称柯基犬) 的 19 个 STR 基因座进行遗传多态性研究。 方法 选用犬荧光标记 19 个 STR 基因座复合扩增试剂盒进行 PCR 扩增,并进行统计学分析。 结果 对 212 只纯种柯基犬的19 个基因座的多态性进行统计学分析,经 Hardy-Weinberg 平衡检验,除 PEZ20 基因座外,P 值均大于 0. 05,18 个基因座的累积非父排除率(CPE)为 0. 9998289783352165,累积个体识别率( TDP) 为 0. 999999999999998,平均杂合度和平均多态信息含量分别为 0. 631 和 0. 604。 结论 19 个 STR 基因座中的 18 个(PEZ20 基因座除外) 联合应用可以用于柯基犬的个体识别和亲权鉴定。

关键词: 柯基犬, STR, 遗传多态性, 亲权鉴定, 个体识别